|
Title
|
|
|
|
Generation of Merkel cell polyomavirus virus-like particles and their application to the detection of MCV antibodies
| |
|
Author
|
|
|
|
| |
|
Abstract
|
|
|
|
| The genome of a new human polyomavirus, known as Merkel cell polyomavirus (MCV), has recently been reported to be integrated within the cellular DNA of Merkel cell carcinoma (MCC), a rare human skin cancer. To investigate MCV seroprevalence in the general population, we expressed three different MCV VP1 in insect cells using recombinant baculoviruses. Virus-like particles were obtained with only one of the three VP1 genes. High titer antibodies against VP1 VLPs were detected in mice immunized with MCV VLPs and limited cross-reactivity was observed with BKV and LPV. MCV antibodies were detected in 77% of the general population, with variations according to age. |
| |
|
Language
|
|
|
|
English
| |
|
Source (journal)
|
|
|
|
Journal of clinical microbiology. - Washington, D.C.
| |
|
Publication
|
|
|
|
Washington, D.C.
:
2010
| |
|
ISSN
|
|
|
|
0095-1137
| |
|
Volume/pages
|
|
|
|
48
:5
(2010)
, p. 1767-1770
| |
|
ISI
|
|
|
|
000277356600040
| |
|
Full text (Publisher's DOI)
|
|
|
|
| |
|