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Title
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Generation of Merkel cell polyomavirus virus-like particles and their application to the detection of MCV antibodies
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Author
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Abstract
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| The genome of a new human polyomavirus, known as Merkel cell polyomavirus (MCV), has recently been reported to be integrated within the cellular DNA of Merkel cell carcinoma (MCC), a rare human skin cancer. To investigate MCV seroprevalence in the general population, we expressed three different MCV VP1 in insect cells using recombinant baculoviruses. Virus-like particles were obtained with only one of the three VP1 genes. High titer antibodies against VP1 VLPs were detected in mice immunized with MCV VLPs and limited cross-reactivity was observed with BKV and LPV. MCV antibodies were detected in 77% of the general population, with variations according to age. |
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Language
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English
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Source (journal)
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Journal of clinical microbiology. - Washington, D.C.
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Publication
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Washington, D.C.
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2010
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ISSN
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0095-1137
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DOI
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10.1128/JCM.01691-09
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Volume/pages
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48
:5
(2010)
, p. 1767-1770
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ISI
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000277356600040
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Full text (Publisher's DOI)
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