Title
Generation of Merkel cell polyomavirus virus-like particles and their application to the detection of MCV antibodiesGeneration of Merkel cell polyomavirus virus-like particles and their application to the detection of MCV antibodies
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Research group
Molecular Imaging, Pathology, Radiotherapy & Oncology (MIPRO)
Publication type
article
Publication
Washington, D.C.,
Subject
Human medicine
Source (journal)
Journal of clinical microbiology. - Washington, D.C.
Volume/pages
48(2010):5, p. 1767-1770
ISSN
0095-1137
ISI
000277356600040
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
The genome of a new human polyomavirus, known as Merkel cell polyomavirus (MCV), has recently been reported to be integrated within the cellular DNA of Merkel cell carcinoma (MCC), a rare human skin cancer. To investigate MCV seroprevalence in the general population, we expressed three different MCV VP1 in insect cells using recombinant baculoviruses. Virus-like particles were obtained with only one of the three VP1 genes. High titer antibodies against VP1 VLPs were detected in mice immunized with MCV VLPs and limited cross-reactivity was observed with BKV and LPV. MCV antibodies were detected in 77% of the general population, with variations according to age.
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