Title
Ethyl glucuronide determination in meconium and hair by hydrophilic interaction liquid chromatographytandem mass spectrometryEthyl glucuronide determination in meconium and hair by hydrophilic interaction liquid chromatographytandem mass spectrometry
Author
Faculty/Department
Faculty of Sciences. Chemistry
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy
Research group
Toxicological Centre
Department of Chemistry
Publication type
article
Publication
Lausanne,
Subject
Biology
Pharmacology. Therapy
Source (journal)
Forensic science international. - Lausanne
Volume/pages
196(2010):1/3, p. 121-127
ISSN
0379-0738
ISI
000274866500024
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Ethyl glucuronide (EtG) detection in non-conventional matrices, such as hair and meconium, can provide useful information on alcohol abuse over a long time frame, for example during pregnancy or after a withdrawal treatment. This study reports on the development, validation and application of a new hydrophilic interaction liquid chromatographytandem mass spectrometry (HILICMS/MS) method for the analysis of EtG in meconium and hair. For each matrix, the sample preparation and the chromatographic separation were thoroughly optimised. Additionally, experiments with reversed-phase liquid chromatography were also performed in the development stages. Analyses were carried out using a Phenomenex Luna HILIC column (150 mm × 3 mm, 5 μm) and a mobile phase composed by ammonium acetate 2 mM and acetonitrile, in gradient. Different SPE cartridges (Oasis MAX, Oasis WAX, aminopropyl silica) and solvents were tested in order to obtain the highest recoveries and cleanest extracts. Optimal results were obtained for meconium with aminopropyl cartridges, while for hair an incubation of 16 h with 2 mL of water and acetonitrile (50/50, v/v) provided good results. The analytical method was validated for both matrices (meconium and hair) by assessing linearity, precision, accuracy, recovery and limit of quantification. The calibration curve concentrations ranged from 50 to 1200 pg/mg for meconium and from 20 to 1000 pg/mg for hair. Real meconium and hair samples were analyzed and results were consistent with literature.
E-info
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