Publication
Title
2D-DIGE reveals changes in wheat xylanase inhibitor protein families due to **Fusarium graminearum** infection and grain development
Author
Abstract
Wheat contains three different classes of proteinaceous xylanase inhibitors (XIs), i.e. Triticum aestivum xylanase inhibitors (TAXI), xylanase inhibiting proteins (XIP), and thaumatin-like xylanase inhibitors (TLXI), which are believed to act as a defensive barrier against phytopathogenic attack. In the absence of relevant data in wheat kernels, we here examined the response of the different members of the XI protein population to infection with a Tri5 mutantof Fusarium graminearum, the wild type of which is one of the most important wheat ear pathogens, in early developing wheat grain. Wheat ears were inoculated at anthesis, analyzed using 2D-DIGE and multivariate analysis at 5, 15, and 25 days post anthesis (DPA) and compared to control samples. Distinct abundance patterns could be distinguished for different XI forms in response to infection with F. graminearum Tri5. Some (iso)forms were up-regulated, while others were down-regulated. This pathogen specific regulation of proteins was mostly visible at 5 DPA and levelled off in the samples situated further from the inoculation point. Furthermore, it was shown that most identified TAXI- and XIP-type XI (iso)forms significantly increased in abundance from the milky (15 DPA) to the soft dough stages (25 DPA) on a per kernel basis, although the extent of increase differed greatly. Non-glycosylated XIP forms increased more strongly than their glycosylated counterparts.
Language
English
Source (journal)
Proteomics. - Weinheim
Publication
Weinheim : 2010
ISSN
1615-9853
Volume/pages
10:12(2010), p. 2303-2319
ISI
000279426300007
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 19.04.2010
Last edited 20.06.2017
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