Title
Phytochemical and biological investigations of **Elaeodendron schlechteranum** Phytochemical and biological investigations of **Elaeodendron schlechteranum**
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy
Publication type
article
Publication
Lausanne ,
Subject
Pharmacology. Therapy
Source (journal)
Journal of ethnopharmacology. - Lausanne
Volume/pages
129(2010) :3 , p. 319-326
ISSN
0378-8741
ISI
000279439300006
Carrier
E
Target language
Dutch (dut)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Aim of the study Elaeodendron schlechteranum (Loes.) Loes. is a shrub or tree belonging to the family Celastraceae. In Tanzania, in addition to ethnopharmacological claims in treating various non-infectious diseases, the root and stem bark powder is applied on septic wounds, and the leaf paste is used for treatment of boils and carbuncles. The aim of this study was to identify the putative active constituents of the plant. Materials and methods Dried and powdered root bark was extracted and subjected to bioassay-guided fractionation, based on antibacterial, antiparasitic and anti-HIV activity. Isolated compounds were identified by spectroscopic methods, and evaluated for biological activity. Results and conclusions Bioassay-guided isolation led to the identification of tingenin B (22β-hydroxytingenone) as the main antibacterial constituent. It was active against Bacillus cereus, Staphylococcus aureus and Escherichia coli (IC50 < 0.25 μg/mL). Furthermore, antiparasitic activity was observed against Trypanosoma cruzi (IC50 < 0.25 μg/mL), Trypanosoma brucei (<0.25 μg/mL), Leishmania infantum (0.51 μg/mL), and Plasmodium falciparum (0.36 μg/mL). Tingenin B was highly cytotoxic to MRC-5 cells (CC50 0.45 μg/mL), indicating a poor selectivity. Two inactive triterpenes, 3β,29-dihydroxyglutin-5-ene and cangoronine methyl ester were also obtained. Phytochemical investigation of the anti-HIV active fractions led to the isolation and identification of three phenolic compounds, namely 4′-O-methylepigallocatechin, 4′-O-methylgallocatechin, and a new procyanidin dimer, i.e. 4′,4triple prime-di-O-methyl-prodelphinidin B4 or 4′-O-methylgallocatechin-(4α → 8)-4′-O-methylepigallocatechin. However, none of these showed anti-HIV activity.
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