Title
**T. cruzi** OligoC-TesT: a simplified and standardized polymerase chain reaction format for diagnosis of Chagas disease **T. cruzi** OligoC-TesT: a simplified and standardized polymerase chain reaction format for diagnosis of Chagas disease
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
Subject
Human medicine
Source (journal)
PLoS neglected tropical diseases
Volume/pages
3(2009) :6 , p. e450,1-e450,8
ISSN
1935-2727
ISI
000268401000020
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Background PCR has evolved into one of the most promising tools for T. cruzi detection in the diagnosis and control of Chagas disease. However, general use of the technique is hampered by its complexity and the lack of standardization. Methodology We here present the development and phase I evaluation of the T. cruzi OligoC-TesT, a simple and standardized dipstick format for detection of PCR amplified T. cruzi DNA. The specificity and sensitivity of the assay were evaluated on blood samples from 60 Chagas non-endemic and 48 endemic control persons and on biological samples from 33 patients, 7 reservoir animals, and 14 vectors collected in Chile. Principal Findings The lower detection limits of the T. cruzi OligoC-TesT were 1 pg and 1 to 10 fg of DNA from T. cruzi lineage I and II, respectively. The test showed a specificity of 100% (95% confidence interval [CI]: 96.6%100%) on the control samples and a sensitivity of 93.9% (95% CI: 80.4%98.3%), 100% (95% CI: 64.6%100%), and 100% (95% CI: 78.5%100%) on the human, rodent, and vector samples, respectively. Conclusions The T. cruzi OligoC-TesT showed high sensitivity and specificity on a diverse panel of biological samples. The new tool is an important step towards simplified and standardized molecular diagnosis of Chagas disease.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/a9d7bc/eaefed79.pdf
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