Title
Development and evaluation of different PCR-based typing methods for discrimination of **Leishmania donovani** isolates from NepalDevelopment and evaluation of different PCR-based typing methods for discrimination of **Leishmania donovani** isolates from Nepal
Author
Faculty/Department
Institute of Development Policy and Management
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Research group
Department of Biomedical Sciences - other
Institute of Development Policy and Management - other
Department of Biomedical Sciences
Publication type
article
Publication
London,
Subject
Human medicine
Source (journal)
Parasitology. - London, 1908, currens
Volume/pages
137(2010):6, p. 947-957
ISSN
0031-1820
1469-8161
ISI
000278673600005
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Introduction Leishmania donovam, the causative agent of visceral leishmaniasis in the Indian subcontinent, has been reported to be genetically homogeneous In order to support ongoing initiatives to eliminate the disease, highly discriminative tools arc required for documenting the parasite population and dynamics Methods. Thirty-four clinical isolates of L donovam from Nepal were analysed on the basis of size and restriction endonuclease polymorphisms of PCR amplicons from kinetoplast minicircle DNA, 5 nuclear microsatellites, and nuclear loci encoding glycoprotein 63, cysteine proteinase B, and hydrophilic acylated surface protein B We present and validate a procedure allowing standardized analysis of kDNA finger print patterns Results. Our results show that parasites ale hest discriminated on the basis of kinetoplast minicircle DNA (14 genotypes) and I microsatellite defining 7 genotypes, while the remaining markers discriminated 2 groups or were monomorphic Combination of all nuclear markers revealed 8 genotypes, while extension with kDNA data yielded 18 genotypes Conclusion. We present tools that allow discrimination of closely related L donovam strains circulating in the Teran region of Nepal These can be used to study the micro-epidemiology of parasite populations, determine the geographical origin of in distinguish relapses from re-infection, and monitor the spread of particular variants
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