|
Title
|
|
|
|
Multiplex Amplicon Quantification (MAQ), a fast and efficient method for the simultaneous detection of copy number alterations in neuroblastoma
| |
|
Author
|
|
|
|
| |
|
Abstract
|
|
|
|
| Background Cancer genomes display characteristic patterns of chromosomal imbalances, often with diagnostic and prognostic relevance. Therefore assays for genome-wide copy number screening and simultaneous detection of copy number alterations in specific chromosomal regions are of increasing importance in the diagnostic work-up of tumors. Results We tested the performance of Multiplex Amplicon Quantification, a newly developed low-cost, closed-tube and high-throughput PCR-based technique for detection of copy number alterations in regions with prognostic relevance for neuroblastoma. Comparison with array CGH and the established Multiplex Ligation-dependent Probe Amplification method on 52 neuroblastoma tumors showed that Multiplex Amplicon Quantification can reliably detect the important genomic aberrations. Conclusion Multiplex Amplicon Quantification is a low-cost and high-throughput PCR-based technique that can reliably detect copy number alterations in regions with prognostic relevance for neuroblastoma. |
| |
|
Language
|
|
|
|
English
| |
|
Source (journal)
|
|
|
|
BMC genomics. - London
| |
|
Publication
|
|
|
|
London
:
2010
| |
|
ISSN
|
|
|
|
1471-2164
| |
|
Volume/pages
|
|
|
|
11
(2010)
, p. 298,1-298,8
| |
|
ISI
|
|
|
|
000278374400001
| |
|
Full text (Publisher's DOI)
|
|
|
|
| |
|
Full text (open access)
|
|
|
|
| |
|