Title
Expression characteristics of potential biomarker genes in Tra catfish, **Pangasianodon hypophthalmus**, exposed to trichlorfon Expression characteristics of potential biomarker genes in Tra catfish, **Pangasianodon hypophthalmus**, exposed to trichlorfon
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
Oxford ,
Subject
Biology
Source (journal)
Comparative biochemistry and physiology: D: genomics and proteomics. - Oxford
Volume/pages
5(2010) :3 , p. 207-216
ISSN
1744-117X
ISI
000281314400004
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Trichlorfon (TRC) is the most common organophosphorous insecticide used in aquaculture practices in Southeast Asian countries. Indiscriminate use of TRC can either damage or alter the enzymatic and hormonal activities in the living organisms. In this present study, therefore, toxicogenomic analyses using real time PCR was used to characterize expression levels of various genes in Pangasianodon hypophthalmus after exposure to three concentrations, the 96 h 1/100LC50 (0.01 mg/L), the 96 h 1⁄10LC50 (0.1 mg/L) and the 96 h 1⁄2LC50 (0.5 mg/L) of TRC for 6 h, 24 h, 96 h, 7 days, 14 days, 28 days and 56 days respectively. The expression kinetics of stress and other cellular toxicity representative genes such as heat shock protein70 (HSP70), growth hormone, acetylcholinesterase (AChE), trypsinogen, cytochrome P4501B (CYP1B) and cytochrome oxidase subunit 1 (COI) were investigated in liver and gills. TRC at a level of 0.1 mg/L and 0.5 mg/L induced a time and dose-dependent increase in the expression of the HSP70, COI and CYPIB while the transcript level of AChE, growth hormone and trypsinogen were significantly down-regulated. These results could permit to develop a molecular biomarker system which can be applied as a first-tier method of identifying contaminant exposure before effects at population level occur.
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