|
Title
|
|
|
|
Prolyl oligopeptidase of **Trypanosoma brucei** hydrolyzes native collagen, peptide hormones and is active in the plasma of infected mice
| |
|
Author
|
|
|
|
| |
|
Abstract
|
|
|
|
| Proteases playimportantrolesinmanybiologicalprocessesofparasites,includingtheirhostinteractions.Insleepingsickness, Trypanosoma brucei proteases releasedintothehostbloodstreamcouldhydrolyzehostfactors,suchashormones,contributingtothedevelopmentofthe diseasessymptoms.Inthisstudy,wepresenttheidentificationofthe T. brucei prolyl oligopeptidasegene( poptb) andthecharacterizationofits corresponding enzyme,POPTb.SecondarystructurepredictionsofPOPTbshowastructuralcompositionhighlysimilartootherPOPs. Recombinant POPTbproducedin E. coli was activeandhighlysensitivetoinhibitorsof Trypanosoma cruzi POP Tc80.Theseinhibitors,which prevent T. cruzi entry intonon-phagocyticcells,arrestedgrowthofthe T. brucei bloodstream forminadose-dependentmanner.POPTb hydrolyzes peptidehormonescontainingProorAlaattheP1positionataslightlyalkalinepH,andalsocleavestypeIcollagen in vitro and nativecollagenpresentinratmesentery.Furthermore,POPTbisreleasedintothebloodstreamof T. brucei infected micewhereitremains active.ThesedatasuggestthatPOPTbmightcontributetothepathogenesisofsleepingsickness. |
| |
|
Language
|
|
|
|
English
| |
|
Source (journal)
|
|
|
|
Microbes and infection / Institut Pasteur [Paris] - Paris, 1999, currens
| |
|
Publication
|
|
|
|
Paris
:
Elsevier
,
2010
| |
|
ISSN
|
|
|
|
1286-4579
[print]
1769-714X
[online]
| |
|
Volume/pages
|
|
|
|
12
:6
(2010)
, p. 457-466
| |
|
ISI
|
|
|
|
000279044800005
| |
|
Full text (Publisher's DOI)
|
|
|
|
| |
|