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Title
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Measurement of carboxypeptidase U (active thrombin-activatable fibrinolysis inhibitor) in plasma: Challenges overcome by a novel selective assay
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Author
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Abstract
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| This article introduces a novel assay for the measurement of carboxypeptidase U (CPU) in plasma using the selective CPU substrate Bz-o-cyano-Phe-Arg (N-benzoyl-ortho-cyano-phenylalanyl-arginine), thereby limiting the interference of plasma carboxypeptidase N (CPN) as well as the intrinsic activity of procarboxypeptidase U (proCPU). A limit of detection of 0.05 U/L (10 pM) was reached. In addition, the current assay has the advantage of being easy to perform and shows excellent linearity and variability, rendering it a useful tool in the screening of samples for the presence of CPU in several patient populations and encouraging in-depth exploration of the pathophysiological role of the proCPU/CPU system. |
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Language
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English
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Source (journal)
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Analytical biochemistry. - New York, N.Y.
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Publication
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New York, N.Y.
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2010
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ISSN
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0003-2697
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Volume/pages
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403
:1/2
(2010)
, p. 114-116
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ISI
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000278793800016
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Full text (Publisher's DOI)
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