|
Title
|
|
|
|
Measurement of carboxypeptidase U (active thrombin-activatable fibrinolysis inhibitor) in plasma: Challenges overcome by a novel selective assay
| |
|
Author
|
|
|
|
| |
|
Abstract
|
|
|
|
| This article introduces a novel assay for the measurement of carboxypeptidase U (CPU) in plasma using the selective CPU substrate Bz-o-cyano-Phe-Arg (N-benzoyl-ortho-cyano-phenylalanyl-arginine), thereby limiting the interference of plasma carboxypeptidase N (CPN) as well as the intrinsic activity of procarboxypeptidase U (proCPU). A limit of detection of 0.05 U/L (10 pM) was reached. In addition, the current assay has the advantage of being easy to perform and shows excellent linearity and variability, rendering it a useful tool in the screening of samples for the presence of CPU in several patient populations and encouraging in-depth exploration of the pathophysiological role of the proCPU/CPU system. |
| |
|
Language
|
|
|
|
English
| |
|
Source (journal)
|
|
|
|
Analytical biochemistry. - New York, N.Y.
| |
|
Publication
|
|
|
|
New York, N.Y.
:
2010
| |
|
ISSN
|
|
|
|
0003-2697
| |
|
DOI
|
|
|
|
10.1016/J.AB.2010.03.045
| |
|
Volume/pages
|
|
|
|
403
:1/2
(2010)
, p. 114-116
| |
|
ISI
|
|
|
|
000278793800016
| |
|
Full text (Publisher's DOI)
|
|
|
|
| |
|