Publication
Title
Study on the loss of nucleoside mono-, di- and triphosphates and phosphorylated peptides to a metal-free LCMS hardware
Author
Abstract
In our earlier LCMS experiments on the analysis of phosphocompounds like nucleotides (mono-, di- and triphosphate) and phosphopeptides and in literature, low sensitivity and severe losses of analyte to the instrumental setup were observed. Since we noticed that the stainless steel parts of the setup (e.g., the electrospray needle) adsorbed important quantities of the phosphorylated analytes, we made a LCESI setup without metallic surfaces. The first results were disappointing since also the fused silica surface of the LC-ESI coupler adsorbed part of the nucleotides and phosphopeptides injected in flow analysis experiments. We present experiments documenting the contribution of the different components of the setup. A number of potential solutions to the adsorption problem are proposed and tested. Only dimethyldichlorosilane deactivation of fused silica capillaries gave satisfactory results as adsorption of nucleotides and phosphopeptide was minimised.
Language
English
Source (journal)
International journal of mass spectrometry. - Amsterdam
Publication
Amsterdam : 2011
ISSN
1387-3806
Volume/pages
304:2/3(2011), p. 83-90
ISI
000293108100004
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 12.10.2010
Last edited 26.11.2017
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