Title
The heme pocket of the globin domain of the globin-coupled sensor of **Geobacter sulfurreducens**: an EPR study The heme pocket of the globin domain of the globin-coupled sensor of **Geobacter sulfurreducens**: an EPR study
Author
Faculty/Department
Faculty of Sciences. Physics
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
New York, N.Y. ,
Subject
Chemistry
Biology
Human medicine
Source (journal)
Journal of inorganic biochemistry. - New York, N.Y.
Volume/pages
104(2010) :10 , p. 1022-1028
ISSN
0162-0134
ISI
000281525200002
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
The globin-coupled sensor (GCS) of Geobacter sulfurreducens is unique amongst GCSs in that its signalling domain is a transmembrane domain with yet unknown function. In the present work we use X-band continuous-wave and pulsed electron paramagnetic resonance (EPR) to investigate the ferric form of the globin domain of the G. sulfurreducens GCS (GsGCS162) at pH 8.5. This form shows a unique bis-histidine coordination of the heme with the F8His and E11His. In contrast with previous crystal structure data, where three conformers of the heme structure were identified, ferric GsGCS162 assumes only one conformation in frozen solution. The EPR data of ferric GsGCS162 are compared in detail with those of other bis-histidine coordinated globins, including other GCS systems.
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