Publication
Title
The heme pocket of the globin domain of the globin-coupled sensor of **Geobacter sulfurreducens**: an EPR study
Author
Abstract
The globin-coupled sensor (GCS) of Geobacter sulfurreducens is unique amongst GCSs in that its signalling domain is a transmembrane domain with yet unknown function. In the present work we use X-band continuous-wave and pulsed electron paramagnetic resonance (EPR) to investigate the ferric form of the globin domain of the G. sulfurreducens GCS (GsGCS162) at pH 8.5. This form shows a unique bis-histidine coordination of the heme with the F8His and E11His. In contrast with previous crystal structure data, where three conformers of the heme structure were identified, ferric GsGCS162 assumes only one conformation in frozen solution. The EPR data of ferric GsGCS162 are compared in detail with those of other bis-histidine coordinated globins, including other GCS systems.
Language
English
Source (journal)
Journal of inorganic biochemistry. - New York, N.Y.
Publication
New York, N.Y. : 2010
ISSN
0162-0134
Volume/pages
104:10(2010), p. 1022-1028
ISI
000281525200002
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 20.10.2010
Last edited 05.08.2017
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