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Title
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The brown shrimp (**Crangon crangon** L.) ecdysteroid receptor complex: cloning, structural modeling of the ligand-binding domain and functional expression in an EcR-deficient **Drosophila** cell line
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Author
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Abstract
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| cDNAs encoding ecdysteroid receptor (EcR) and retinoid X receptor (RXR) were cloned and sequenced from brown shrimp Crangon crangon (Crustacea: Decapoda), a common faunal species and commercially important in the North-West European coastal waters. A 3D model of the ligand-binding domain (LBD) of EcR was created and docking of ponasterone A (PonA) was simulated in silico. Finally, we report the transfection of expression plasmids for these receptors in the mutant Drosophila L57-3-11 cell line. Through an ecdysteroid responsive reporter assay we clearly prove the functionality of shrimp ecdysteroid receptor in the transfected L57-3-11 cell line. Our results indicate that the Drosophila L57-3-11 cell line and in silico LBD modeling can be used to study the function of crustacean ecdysteroid receptors and be applied to assess endocrine disrupting effects on non-target crustacean species. |
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Language
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English
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Source (journal)
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General and comparative endocrinology. - New York
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Publication
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New York
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2010
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ISSN
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0016-6480
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DOI
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10.1016/J.YGCEN.2010.05.007
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Volume/pages
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168
:3
(2010)
, p. 415-423
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ISI
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000281126700014
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Full text (Publisher's DOI)
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