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Title
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Controlling -activated channels with models of buffering in Purkinje cells
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Author
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Abstract
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| Intracellular Ca2+ concentrations play a crucial role in the physiological interaction between Ca2+ channels and Ca2+-activated K+ channels. The commonly used model, a Ca2+ pool with a short relaxation time, fails to simulate interactions occurring at multiple time scales. On the other hand, detailed computational models including various Ca2+ buffers and pumps can result in large computational cost due to radial diffusion in large compartments, which may be undesirable when simulating morphologically detailed Purkinje cell models. We present a method using a compensating mechanism to replace radial diffusion and compared the dynamics of different Ca2+ buffering models during generation of a dendritic Ca2+ spike in a single compartment model of a PC dendritic segment with Ca2+ channels of P- and T-type and Ca2+-activated K+ channels of BK- and SK-type. The Ca2+ dynamics models used are (1) a single Ca2+ pool; (2) two Ca2+ pools, respectively, for the fast and slow transients; (3) detailed Ca2+ dynamics with buffers, pump, and diffusion; and (4) detailed Ca2+ dynamics with buffers, pump, and diffusion compensation. Our results show that detailed Ca2+ dynamics models have significantly better control over Ca2+-activated K+ channels and lead to physiologically more realistic simulations of Ca2+ spikes and bursting. Furthermore, the compensating mechanism largely eliminates the effect of removing diffusion from the model on Ca2+ dynamics over multiple time scales. |
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Language
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English
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Source (journal)
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The Cerebellum. - -
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Publication
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2012
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ISSN
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1473-4222
[print]
1473-4230
[online]
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DOI
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10.1007/S12311-010-0224-3
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Volume/pages
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11
:3
(2012)
, p. 681-693
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ISI
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000307291500008
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Full text (Publisher's DOI)
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Full text (open access)
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