Title
Controlling <tex>$Ca^{2+}$</tex>-activated <tex>$K^{+}$</tex> channels with models of <tex>$Ca^{2+}$</tex> buffering in Purkinje cells Controlling <tex>$Ca^{2+}$</tex>-activated <tex>$K^{+}$</tex> channels with models of <tex>$Ca^{2+}$</tex> buffering in Purkinje cells
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
Subject
Human medicine
Source (journal)
Cerebellum
Volume/pages
11(2012) :3 , p. 681-693
ISSN
1473-4222
ISI
000307291500008
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Intracellular Ca2+ concentrations play a crucial role in the physiological interaction between Ca2+ channels and Ca2+-activated K+ channels. The commonly used model, a Ca2+ pool with a short relaxation time, fails to simulate interactions occurring at multiple time scales. On the other hand, detailed computational models including various Ca2+ buffers and pumps can result in large computational cost due to radial diffusion in large compartments, which may be undesirable when simulating morphologically detailed Purkinje cell models. We present a method using a compensating mechanism to replace radial diffusion and compared the dynamics of different Ca2+ buffering models during generation of a dendritic Ca2+ spike in a single compartment model of a PC dendritic segment with Ca2+ channels of P- and T-type and Ca2+-activated K+ channels of BK- and SK-type. The Ca2+ dynamics models used are (1) a single Ca2+ pool; (2) two Ca2+ pools, respectively, for the fast and slow transients; (3) detailed Ca2+ dynamics with buffers, pump, and diffusion; and (4) detailed Ca2+ dynamics with buffers, pump, and diffusion compensation. Our results show that detailed Ca2+ dynamics models have significantly better control over Ca2+-activated K+ channels and lead to physiologically more realistic simulations of Ca2+ spikes and bursting. Furthermore, the compensating mechanism largely eliminates the effect of removing diffusion from the model on Ca2+ dynamics over multiple time scales.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/19e3b1/2522.pdf
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