Publication
Title
Unravelling the antigenic landscape of the HIV-1 subtype A envelope of an individual with broad cross-neutralizing antibodies using phage display peptide libraries
Author
Abstract
Broad cross-neutralizing antibodies from persons infected with HIV-1 target a variety of epitopes. Identification of these HIV-1 epitopes may result in an optimal panel of antigenic peptides to be included in a prophylactic vaccine. Phage display peptide libraries were used to unravel the antigenic landscape of an individual (ITM1) infected with HIV-1 subtype A with broad cross-neutralizing antibodies. A stringent selection strategy resulted in the identification of 60 unique HIV-1 peptide phage, which were subjected to sequence analysis and mapped onto the ITM1 envelope sequences. Four groups of peptide phages were found: the first group (n = 11) were similar with the tip of the V3 loop (KxxHxGPxxxF); the second group (n = 11) represented the gp41 principal immunodominant domain (CxGxLxCTxNxP); the third group (n = 16) could be localized in the V2 loop (KxxxHxxxY); and the fourth group (n = 22) mimicked a conformational epitope (HxxS/TNxK). All but the V2-binding antibodies were conserved over the 11 years of follow-up. A neutralization inhibition assay revealed the contribution of the V3 antibodies to the neutralizing capacity of the ITM1 plasma. Overall, the ITM1 immunogenic landscape was mapped and a part of the origin of this broad cross-neutralizing activity was demonstrated.
Language
English
Source (journal)
Journal of virological methods. - Amsterdam
Publication
Amsterdam : 2010
ISSN
0166-0934
Volume/pages
169:1(2010), p. 95-102
ISI
000282398600015
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 12.01.2011
Last edited 18.12.2017
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