Publication
Title
The identification of cellular targets of 17$\beta$ estradiol using a lytic (T7) cDNA phage display approach
Author
Abstract
 To unravel the mechanism of action of chemical compounds, it is crucial to know their cellular targets. A novel in vitro tool that can be used as a fast, simple and cost effective alternative is cDNA phage display. This tool is used in our study to select cellular targets of 17β estradiol (E2). It was possible to select two potential cellular targets of E2 out of the T7 Select Human Breast cDNA phage library. The selected cellular targets, autophagy/beclin-1 regulator 1 (beclin 1) and ATP synthase F0 subunit 6 (ATP6) have so far been unknown as binding proteins of E2. To confirm the E2 binding properties of these selected proteins, surface plasmon resonance (SPR) was used. With SPR the Kd values were determined to be 0.178 ± 0.031 and 0.401 ± 0.142 nM for the ATP6 phage and beclin 1 phage, respectively. These Kd values in the low nM range verify that the selected cellular proteins are indeed binding proteins for E2. The selection and identification of these two potential cellular targets of E2, can enhance our current understanding of its mechanism of action. This illustrates the potential of lytic (T7) cDNA phage display in toxicology, to provide important information about cellular targets of chemical compounds.
Language
English
Source (journal)
Toxicology in vitro. - Oxford
Publication
Oxford : 2011
ISSN
0887-2333
Volume/pages
25:1(2011), p. 388-393
ISI
000287010600046
Full text (Publisher's DOI)
UAntwerpen
 Faculty/Department Research group Publication type Subject Affiliation Publications with a UAntwerp address