Title The identification of cellular targets of 17$\beta$ estradiol using a lytic (T7) cDNA phage display approachThe identification of cellular targets of 17$\beta$ estradiol using a lytic (T7) cDNA phage display approach Author Van Dorst, Bieke Mehta, Jaytry Rouah-Martin, Elsa de Coen, Wim Blust, Ronny Robbens, Johan Faculty/Department Faculty of Sciences. Biology Research group Systemic Physiological and Ecotoxicological Research (SPHERE) Department of Biology Publication type article Publication 2011Oxford, 2011 Subject Biology Source (journal) Toxicology in vitro. - Oxford Volume/pages 25(2011):1, p. 388-393 ISSN 0887-2333 ISI 000287010600046 Carrier E Target language English (eng) Full text (Publishers DOI) Affiliation University of Antwerp Abstract To unravel the mechanism of action of chemical compounds, it is crucial to know their cellular targets. A novel in vitro tool that can be used as a fast, simple and cost effective alternative is cDNA phage display. This tool is used in our study to select cellular targets of 17β estradiol (E2). It was possible to select two potential cellular targets of E2 out of the T7 Select Human Breast cDNA phage library. The selected cellular targets, autophagy/beclin-1 regulator 1 (beclin 1) and ATP synthase F0 subunit 6 (ATP6) have so far been unknown as binding proteins of E2. To confirm the E2 binding properties of these selected proteins, surface plasmon resonance (SPR) was used. With SPR the Kd values were determined to be 0.178 ± 0.031 and 0.401 ± 0.142 nM for the ATP6 phage and beclin 1 phage, respectively. These Kd values in the low nM range verify that the selected cellular proteins are indeed binding proteins for E2. The selection and identification of these two potential cellular targets of E2, can enhance our current understanding of its mechanism of action. This illustrates the potential of lytic (T7) cDNA phage display in toxicology, to provide important information about cellular targets of chemical compounds. E-info http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000287010600046&DestLinkType=RelatedRecords&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000287010600046&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000287010600046&DestLinkType=CitingArticles&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848 Handle