Determination of bisphenol-A, triclosan and tetrabromobisphenol-A in human serum using SPE and GG/ECNI-MSDetermination of bisphenol-A, triclosan and tetrabromobisphenol-A in human serum using SPE and GG/ECNI-MS
Faculty of Sciences. Biology
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy
Systemic Physiological and Ecotoxicological Research (SPHERE)
69(2007), p. 2995-2998
University of Antwerp
The present study focussed on the optimization and validation of a sensitive method for the determination of bisphenol A (BPA), triclosan (TCS) and tetrabromobisphenol A (TBBPA) in human serum. The method implied protein denaturation with formic acid, followed by solid phase extraction on OASIS® HLB cartridge, the analytes being eluted with methanol (MeOH). A further clean-up on Florisil® was applied and the analytes were collected in a fraction of 9 mL MeOH-dichloromethane (DCM) (5/1, v/v) followed by derivatization with pentafluoropropionic acid (PFPA). To eliminate acid by-products formed during derivatization, a liquid-liquid extraction with hexane-DCM (4/1, v/v) and K2CO3 3% solution was applied prior analysis of the final concentrated extract by GCECNI-MS using specific ions for the PFP-derivatives. The absolute recovery values of the analytes were ranged between 71 and 85% for two levels of concentration (0.16 and 14 ng/mL serum). Furthermore, the optimized method was applied for the determination of these compounds in 21 Belgian human serum samples and they were found at detection frequencies higher than 70%. The analytes were measured at concentrations up to 3.8 ng/mL for TCS and BPA, with TBBPA being found at lower concentrations, up to 0.18 ng/mL serum.