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Title
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Apoplastic alkalinization is instrumental for the inhibition of cell elongation in the **Arabidopsis thaliana** root by the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC)
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Author
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Abstract
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| In Arabidopsis thaliana (Col-0) roots the so called zone of cell elongation comprises two clearly different domains: the transition zone, a post-meristematic region (approximately 200 to 450 µm proximal of the root tip) with a low rate of elongation, and a fast elongation zone, the adjacent proximal region (450 µm away from the root tip up to the first root hair) with a high rate of elongation. In this study, the surface pH was measured in both zones using the microelectrode ion flux estimation (MIFE) technique. The surface pH is highest in the apical part of the transition zone and is lowest at the basal part of the fast elongation zone. Fast cell elongation is inhibited within minutes by the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) and concomitantly apoplastic alkalinisation occurs in the affected root zone. Fusicoccin (FC), an activator of the plasma membrane (PM) H+-ATPase, can partially rescue this inhibition of cell elongation, whereas the inhibitor N, N-dicyclohexylcarbodiimide (DCCD) does not further reduce the maximal cell length. MIFE experiments with auxin-mutants lead to the final conclusion that control of the activity state of PM H+-ATPases is one of the mechanisms by which ethylene, via auxin, affects the final cell length in the root. |
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Language
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English
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Source (journal)
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Plant physiology. - Rockville, Md
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Publication
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Rockville, Md
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2011
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ISSN
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0032-0889
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DOI
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10.1104/PP.110.168476
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Volume/pages
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155
:4
(2011)
, p. 2049-2055
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ISI
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000289095500051
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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