Title
Validation of an adiponectin immunoassay in human skeletal muscle biopsies Validation of an adiponectin immunoassay in human skeletal muscle biopsies
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
Amsterdam ,
Subject
Human medicine
Source (journal)
Journal of immunological methods. - Amsterdam
Volume/pages
62(2010) :1/2 , p. 209-212
ISSN
0022-1759
ISI
000284749100029
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Adiponectin is an insulin-sensitizing adipocytokine that circulates in plasma as multimeric isoforms, including trimers, hexamers and high molecular weight complexes. Although adiponectin multimers have previously been measured by Western blot, this remains a relatively delicate technique that is often hampered by high background or multiple bands. We validated a commercially available ELISA, designed to measure total (low, middle and high molecular weight) human adiponectin concentration in cell culture supernatants, serum and plasma, for its proper use in skeletal muscle biopsies obtained in patients with chronic heart failure and healthy controls.
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