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Title
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Axial ligation of the high-potential heme center in an **Arabidopsis** cytochrome b561
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Author
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Abstract
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| Arabidopsis has four putative, di-heme cytochrome b561 proteins, including one localized in the tonoplast (TCytb). From a comparative electron paramagnetic resonance (EPR), UVVis absorption and resonance Raman study, on wild type, H83A/H156A-TCytb and H83L/H156L-TCytb double mutants, it follows that the H83 and H156 residues are binding one of the two hemes. These measurements show that the high-potential heme site is situated at the cytoplasmic side of the membrane and allow the unambiguous differentiation between two models on the heme localization in cytochrome b561 proteins. |
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Language
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English
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Source (journal)
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FEBS letters: for the rapid publication of short reports in biochemistry, biophysics and molecular biology / Federation of European Biochemical Societies. - Amsterdam
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Publication
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Amsterdam
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2011
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ISSN
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0014-5793
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DOI
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10.1016/J.FEBSLET.2011.01.006
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Volume/pages
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585
:3
(2011)
, p. 545-548
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ISI
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000286657700016
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Full text (Publisher's DOI)
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Full text (open access)
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