Title
Properties of deactivation gating currents in shaker channels Properties of deactivation gating currents in shaker channels
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
New York, N.Y. ,
Subject
Physics
Biology
Source (journal)
Biophysical journal. - New York, N.Y.
Volume/pages
100(2011) :5 , p. L28-L30
ISSN
0006-3495
ISI
000288049400002
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
The charge versus voltage relation of voltage-sensor domains shifts in the voltage axis depending on the initial voltage. Here we show that in nonconducting W434F Shaker K+ channels, a large portion of this charge-voltage shift is apparent due to a dramatic slowing of the deactivation gating currents, IgD (with τ up to 80 ms), which develops with a time course of not, vert, similar1.8 s. This slowing in IgD adds up to the slowing due to pore opening and is absent in the presence of 4-aminopyridine, a compound that prevents the last gating step that leads to pore opening. A remaining 1015 mV negative shift in the voltage dependence of both the kinetics and the charge movement persists independently of the depolarizing prepulse duration and remains in the presence of 4-aminopyridine, suggesting the existence of an intrinsic offset in the local electric field seen by activated channels. We propose a new (to our knowledge) kinetic model that accounts for these observations.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/8a113b/5012.pdf
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