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Title
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Denaturing gradient gel electrophoresis of neonatal intestinal microbiota in relation to the development of asthma
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Author
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Abstract
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| Background The extended 'hygiene hypothesis' suggests that the initial composition of the infant gut microbiota is a key determinant in the development of atopic disease. Several studies have demonstrated that the microbiota of allergic and non-allergic infants are different even before the development of symptoms, with a critical time window during the first 6 months of life. The aim of the study was to investigate the association between early intestinal colonisation and the development of asthma in the first 3 years of life using DGGE (denaturing gradient gel electrophoresis). Methods In a prospective birth cohort, 110 children were classified according to the API (Asthma Predictive Index). A positive index included wheezing during the first three years of life combined with eczema in the child in the first years of life or with a parental history of asthma. A fecal sample was taken at the age of 3 weeks and analysed with DGGE using universal and genus specific primers. Results The Asthma Predictive Index was positive in 24/110 (22%) of the children. Using universal V3 primers a band corresponding to a Clostridum coccoides XIVa species was significantly associated with a positive API. A Bacteroides fragilis subgroup band was also significantly associated with a positive API. A final DGGE model, including both bands, allowed correct classification of 73% (80/110) of the cases. Conclusion Fecal colonisation at age 3 weeks with either a Bacteroides fragilis subgroup or a Clostridium coccoides subcluster XIVa species is an early indicator of possible asthma later in life. These findings need to be confirmed in a new longitudinal follow-up study. |
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Language
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English
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Source (journal)
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BMC microbiology. - London
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Publication
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London
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2011
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ISSN
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1471-2180
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DOI
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10.1186/1471-2180-11-68
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Volume/pages
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11
(2011)
, p. 1-7
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Article Reference
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68
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ISI
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000289679900001
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Medium
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E-only publicatie
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Full text (Publisher's DOI)
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Full text (open access)
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