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Title
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Proteome analysis of mouse brain exposed to chronic hypoxia
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Author
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Abstract
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| Objective: Chronic hypoxia exists in many diseases, including cancer. The subject of our study is analysis of molecular pathways affected in the chronically hypoxic mouse brain. Materials and Methods: Using the emPAI protocol, we performed a quantitative proteomic approach to characterize the global proteome in the mouse brain exposed to 7% 02 for 48 hours. Results: Utilizing the emPAI protocol to estimate protein abundance and assign molar concentrations to all proteins, we were able to identify 33 proteins with significant changes in their expression. Conclusion: Deregulated proteins were mainly involved in cell metabolism, apoptosis, Ca2+ signaling, pentose phosphate pathway, 14-3-3 protein mediated signaling cascades and protein degradation. The obtained data will provide some clues for understanding mechanisms with which cells respond and adapt to chronic hypoxia. |
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Language
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English
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Source (journal)
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Yakhteh
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Publication
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2011
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ISSN
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1561-4921
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Volume/pages
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12
:4
(2011)
, p. 503-510
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ISI
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000288889200012
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Full text (publisher's version - intranet only)
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