Title
Proteome analysis of mouse brain exposed to chronic hypoxia Proteome analysis of mouse brain exposed to chronic hypoxia
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
Subject
Human medicine
Source (journal)
Yakhteh
Volume/pages
12(2011) :4 , p. 503-510
ISSN
1561-4921
ISI
000288889200012
Carrier
E
Target language
English (eng)
Affiliation
University of Antwerp
Abstract
Objective: Chronic hypoxia exists in many diseases, including cancer. The subject of our study is analysis of molecular pathways affected in the chronically hypoxic mouse brain. Materials and Methods: Using the emPAI protocol, we performed a quantitative proteomic approach to characterize the global proteome in the mouse brain exposed to 7% 02 for 48 hours. Results: Utilizing the emPAI protocol to estimate protein abundance and assign molar concentrations to all proteins, we were able to identify 33 proteins with significant changes in their expression. Conclusion: Deregulated proteins were mainly involved in cell metabolism, apoptosis, Ca2+ signaling, pentose phosphate pathway, 14-3-3 protein mediated signaling cascades and protein degradation. The obtained data will provide some clues for understanding mechanisms with which cells respond and adapt to chronic hypoxia.
E-info
https://repository.uantwerpen.be/docman/iruaauth/a7030c/a9881f9ce1c.pdf
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