Title
Development of a microarray for **Enchytraeus albidus** (Oligochaeta) : preliminary tool with diverse applicationsDevelopment of a microarray for **Enchytraeus albidus** (Oligochaeta) : preliminary tool with diverse applications
Author
Faculty/Department
Faculty of Sciences. Biology
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Research group
Systemic Physiological and Ecotoxicological Research (SPHERE)
VIB DMG - Applied Molecular Genomics
Publication type
article
Publication
New York, N.Y.,
Subject
Biology
Source (journal)
Environmental toxicology and chemistry. - New York, N.Y.
Volume/pages
30(2011):6, p. 1395-1402
ISSN
0730-7268
ISI
000290825200020
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Standard bioassays allow hazard assessment at the population level, but much remains to be learned about the molecular level response of organisms to stressors. The main aim of this study was the development of a DNA microarray for Enchytraeus albidus, a common soil worm species. Further, this microarray was tested using worms exposed to Cu, phenmedipham, and different soil types. Hybridization onto the developed microarray revealed several genes with homology to known sequences. Genes of interest were confirmed through real-time polymerase chain reaction. It was possible to discriminate between natural and chemical stressors and chemical concentrations. Gene responses were detected under conditions known to have effects in the reproduction of individuals. It was confirmed that the integration of different endpoints improves the assessment process and enhances the understanding of the modes of action of stressors. The chemical stress-induced genes were related to factors such as immune response, stress response, metabolic processes, and/or signal transduction. The present study represents the first step of a gene-level study in the ecologically relevant and standard test species E. albidus. It demonstrates the usefulness of cDNA normalization in the production of cDNA libraries of ecotoxicological standard organisms that are not genome models like E. albidus.
E-info
https://repository.uantwerpen.be/docman/iruaauth/439e79/0e6d1b3d9fa.pdf
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