Publication
Title
Electrochemical study of gelatin as a matrix for the immobilization of horse heart cytochrome **c**
Author
Abstract
The aim of this paper is to emphasize the strength of gelatin as a stable matrix for redox enzymes. Cyclic voltammetry has been applied for a detailed electrochemical study of horse heart cytochrome c (HHC) entrapped in a gelatin matrix immobilized on a gold electrode. The influence of the HHC concentration, the mass percentage of the gelatin and the nature of the gelatin on the electrochemical behaviour of HHC have been described in detail. In addition, attenuated total reflection infrared (ATR-IR) spectroscopy was used to prove the immobilization on a qualitative and conformational level. The thickness of the gelatin film was determined using a non-contact optical profiler. These results open up new perspectives in the development of stable, biocompatible matrices for redox enzymes. The latter has its relevance in the field of biosensor development.
Language
English
Source (journal)
Talanta : the international journal of pure and applied analytical chemistry. - Oxford, 1958, currens
Publication
Oxford : Pergamon, 2010
ISSN
0039-9140 [print]
1873-3573 [online]
Volume/pages
82:5(2010), p. 1980-1985
ISI
000283903300051
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 20.06.2011
Last edited 03.11.2017