Publication
Title
Immobilization of cytochrome **c** on cysteamine-modified gold electrodes with EDC as coupling agent
Author
Abstract
Cyclic voltammetry has been applied for the characterization of cross-linked horse heart cytochrome c (HHC) on cysteamine-modified gold electrodes. The cross-linking, i.e. amide bond formation, between the proteins was achieved by using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) as coupling reagent. The optimal conditions for the formation of the HHC film were determined by varying the HHC concentration. In addition the reproducibility, stability and the influence of the scan rate upon these films were investigated with cyclic voltammetry. The protein film stability in a 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer solution was tested by UV/vis absorption spectroscopy.
Language
English
Source (journal)
Talanta : the international journal of pure and applied analytical chemistry. - Oxford, 1958, currens
Publication
Oxford : Pergamon, 2008
ISSN
0039-9140 [print]
1873-3573 [online]
Volume/pages
76:2(2008), p. 309-313
ISI
000257283100013
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 20.06.2011
Last edited 03.11.2017