Title
Surface characterization of a cross-linked cytochrome c film on cysteamine-modified gold electrodes Surface characterization of a cross-linked cytochrome c film on cysteamine-modified gold electrodes
Author
Faculty/Department
Faculty of Sciences. Chemistry
Publication type
article
Publication
London ,
Subject
Physics
Chemistry
Source (journal)
Surface and interface analysis. - London
Volume/pages
41(2009) :5 , p. 389-393
ISSN
0142-2421
ISI
000265542800007
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
The aim of the present paper is to characterize a cross-linked horse heart cytochrome c (HHC) film on cysteamine-modified gold electrodes. The HHC film was deposited using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) as a coupling agent. Attenuated total reflection infrared (ATR-IR) spectroscopic analyses were performed to characterize the newly formed surface on a qualitative and conformational level. The film thickness was measured using a noncontact optical surface profiler, while quantitative data and information on the heterogeneity of the film were obtained by means of synchrotron radiation X-ray micro fluorescence (SR micro-XRF). Results indicate that, in addition to electrochemical studies, spectroscopic analysis methods are essential to gain insight in the effect of immobilization strategies on protein conformations. The latter is of relevance in the development and optimization of biosensors.
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