Publication
Title
Surface characterization of a cross-linked cytochrome c film on cysteamine-modified gold electrodes
Author
Abstract
The aim of the present paper is to characterize a cross-linked horse heart cytochrome c (HHC) film on cysteamine-modified gold electrodes. The HHC film was deposited using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) as a coupling agent. Attenuated total reflection infrared (ATR-IR) spectroscopic analyses were performed to characterize the newly formed surface on a qualitative and conformational level. The film thickness was measured using a noncontact optical surface profiler, while quantitative data and information on the heterogeneity of the film were obtained by means of synchrotron radiation X-ray micro fluorescence (SR micro-XRF). Results indicate that, in addition to electrochemical studies, spectroscopic analysis methods are essential to gain insight in the effect of immobilization strategies on protein conformations. The latter is of relevance in the development and optimization of biosensors.
Language
English
Source (journal)
Surface and interface analysis. - London
Publication
London : 2009
ISSN
0142-2421
Volume/pages
41:5(2009), p. 389-393
ISI
000265542800007
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 20.06.2011
Last edited 20.09.2017