Publication
Title
Electrochemical determination of hydrogen peroxide using **Rhodobacter capsulatus** cytochrome c peroxidase at a gold electrode
Author
Abstract
We describe the redox behaviour of horse heart cytochrome c (HHC) and Rhodobacter capsulatus cytochrome c peroxidase (RcCCP) at a gold electrode modified with 4,4′-bipyridyl. RcCCP shows no additional oxidation or reduction peaks compared to the electrochemistry of only HHC, which indicates that it most likely binds to HHC and results in a potential downshift of the voltammetric signals of the latter. Furthermore, the electrochemical determination of hydrogen peroxide at a RcCCP/HHC modified gold electrode is shown. The results demonstrate that HHC can substitute for cytochrome c 2, the physiological electron donor. The buffer 2-[4-(2-hydroxyethyl)-piperazinyl]‐ethanesulfonic acid (HEPES) and tris(hydroxymethyl) methylamine (Tris) electrochemically are not as inert as previously believed. They can react with oxygen (radicals) during electrochemical measurements, and the products formed can give rise to additional redox peaks. We therefore also have conducted a voltammetric study on theses buffers.
Language
English
Source (journal)
Microchimica acta: an international journal on micro and trace analysis
Publication
2008
Volume/pages
162:1/2(2008), p. 65-71
ISI
000257213200006
Full text (Publishers DOI)
UAntwerpen
Faculty/Department
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 20.06.2011
Last edited 22.04.2017