Publication
Title
Validated primer set that prevents nuclear DNA sequences of mitochondrial origin co-amplification : a revision based on the New Human Genome Reference Sequence (GRCh37)
Author
Abstract
A new human genome reference sequence GRCh37 was recently generated and made available by the Genome Reference Consortium. Since the prior disposable human reference sequence hg18 was previously used for the mitochondrial DNA primer BLAST validation, a revision of those previously published primer pairs is required. Thus, the aim of this Short Communication is to perform an in silico BLAST test of the published disposable nine primer pairs using the new human reference sequence and to report the pertinent modifications. The new analysis showed that one of the tested primer pairs requires a revision. Therefore, a new validated primer pair, which specifically amplifies the mitochondrial region located between positions 6520 and 9184, is presented.
Language
English
Source (journal)
Electrophoresis. - Weinheim
Publication
Weinheim : 2011
ISSN
0173-0835
Volume/pages
32:6/7(2011), p. 782-783
ISI
000288602000018
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 21.06.2011
Last edited 24.06.2017
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