Title
Validated primer set that prevents nuclear DNA sequences of mitochondrial origin co-amplification : a revision based on the New Human Genome Reference Sequence (GRCh37) Validated primer set that prevents nuclear DNA sequences of mitochondrial origin co-amplification : a revision based on the New Human Genome Reference Sequence (GRCh37)
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
Weinheim ,
Subject
Chemistry
Biology
Human medicine
Source (journal)
Electrophoresis. - Weinheim
Volume/pages
32(2011) :6/7 , p. 782-783
ISSN
0173-0835
ISI
000288602000018
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
A new human genome reference sequence GRCh37 was recently generated and made available by the Genome Reference Consortium. Since the prior disposable human reference sequence hg18 was previously used for the mitochondrial DNA primer BLAST validation, a revision of those previously published primer pairs is required. Thus, the aim of this Short Communication is to perform an in silico BLAST test of the published disposable nine primer pairs using the new human reference sequence and to report the pertinent modifications. The new analysis showed that one of the tested primer pairs requires a revision. Therefore, a new validated primer pair, which specifically amplifies the mitochondrial region located between positions 6520 and 9184, is presented.
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