Publication
Title
The centaurin -1 GTPase-like domain functions as an NTPase
Author
Abstract
Centaurins are a family of proteins that contain GTPase-activating protein domains, with the γ family members containing in addition a GTPase-like domain. Centaurins reside mainly in the nucleus and are known to activate phosphoinositide 3-kinase, a key regulator of cell proliferation, motility and vesicular trafficking. In the present study, using X-ray structural analysis, enzymatic assays and nucleotide-binding studies, we show that, for CENTG1 (centaurin γ-1) the GTPase-like domain has broader trinucleotide specificity. Alterations within the G4 motif of CENTG1 from the highly conserved NKXD found in typical GTPases to TQDR result in the loss of specificity, a lower affinity for the nucleotides and higher turnover rates. These results indicate that the centaurins could be more accurately classified as NTPases and point to alternative mechanisms of cell signalling control.
Language
English
Source (journal)
The biochemical journal. - London, 1984, currens
Publication
London : 2007
ISSN
0264-6021 [print]
1470-8728 [online]
Volume/pages
401:3(2007), p. 679-688
ISI
000243982700008
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 29.06.2011
Last edited 26.06.2017