Title
The centaurin <tex>$\gamma$</tex>-1 GTPase-like domain functions as an NTPase The centaurin <tex>$\gamma$</tex>-1 GTPase-like domain functions as an NTPase
Author
Faculty/Department
Faculty of Sciences. Chemistry
Publication type
article
Publication
London ,
Subject
Chemistry
Biology
Source (journal)
The biochemical journal. - London, 1984, currens
Volume/pages
401(2007) :3 , p. 679-688
ISSN
0264-6021
1470-8728
ISI
000243982700008
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Centaurins are a family of proteins that contain GTPase-activating protein domains, with the γ family members containing in addition a GTPase-like domain. Centaurins reside mainly in the nucleus and are known to activate phosphoinositide 3-kinase, a key regulator of cell proliferation, motility and vesicular trafficking. In the present study, using X-ray structural analysis, enzymatic assays and nucleotide-binding studies, we show that, for CENTG1 (centaurin γ-1) the GTPase-like domain has broader trinucleotide specificity. Alterations within the G4 motif of CENTG1 from the highly conserved NKXD found in typical GTPases to TQDR result in the loss of specificity, a lower affinity for the nucleotides and higher turnover rates. These results indicate that the centaurins could be more accurately classified as NTPases and point to alternative mechanisms of cell signalling control.
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