Title
Application of a proteolysis/mass spectrometry method for investigating the effects of inhibitors on hydroxylase structure Application of a proteolysis/mass spectrometry method for investigating the effects of inhibitors on hydroxylase structure
Author
Faculty/Department
Faculty of Sciences. Chemistry
Publication type
article
Publication
Washington, D.C. ,
Subject
Chemistry
Source (journal)
Journal of medicinal chemistry. - Washington, D.C., 1963, currens
Volume/pages
52(2009) :9 , p. 2799-2805
ISSN
0022-2623
ISI
000265911800018
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Limited proteolysis coupled to matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analyses can be used to screen for compounds that alter protein structure by monitoring stabilizing/destabilizing effects with respect to the rate and nature of proteolysis. When applied to prolyl hydroxylase 2, a key enzyme involved in human oxygen sensing, the method efficiently revealed differential effects on proteolytic stability for structurally similar compounds and for different substrates.
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