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Title
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Application of a proteolysis/mass spectrometry method for investigating the effects of inhibitors on hydroxylase structure
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Author
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Abstract
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| Limited proteolysis coupled to matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analyses can be used to screen for compounds that alter protein structure by monitoring stabilizing/destabilizing effects with respect to the rate and nature of proteolysis. When applied to prolyl hydroxylase 2, a key enzyme involved in human oxygen sensing, the method efficiently revealed differential effects on proteolytic stability for structurally similar compounds and for different substrates. |
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Language
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English
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Source (journal)
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Journal of medicinal chemistry. - Washington, D.C., 1963, currens
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Publication
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Washington, D.C.
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2009
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ISSN
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0022-2623
[print]
1520-4804
[online]
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DOI
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10.1021/JM900285R
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Volume/pages
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52
:9
(2009)
, p. 2799-2805
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ISI
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000265911800018
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Full text (Publisher's DOI)
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