Title
Structure of an Hsp90-Cdc37-Cdk4 complex Structure of an Hsp90-Cdc37-Cdk4 complex
Author
Faculty/Department
Faculty of Sciences. Chemistry
Publication type
article
Publication
Cambridge, Mass. ,
Subject
Chemistry
Source (journal)
Molecular cell. - Cambridge, Mass.
Volume/pages
23(2006) :5 , p. 697-707
ISSN
1097-2765
ISI
000240663400008
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Activation of many protein kinases depends on their interaction with the Hsp90 molecular chaperone system. Recruitment of protein kinase clients to the Hsp90 chaperone system is mediated by the cochaperone adaptor protein Cdc37, which acts as a scaffold, simultaneously binding protein kinases and Hsp90. We have now expressed and purified an Hsp90-Cdc37-Cdk4 complex, defined its stoichiometry, and determined its 3D structure by single-particle electron microscopy. Comparison with the crystal structure of Hsp90 allows us to identify the locations of Cdc37 and Cdk4 in the complex and suggests a mechanism by which conformational changes in the kinase are coupled to the Hsp90 ATPase cycle.
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