Publication
Title
Sensitive colorimetric assay for angiotensin converting enzyme in serum
Author
Abstract
A sensitive colorimetric procedure has been developed for the assay of angiotensin converting enzyme (EC 3.4.15.1) in serum. Serum (10 microL) is incubated for 30 min with hippuryl-glycyl-glycine as described earlier (Clin Chem 28: 1352-1355, 1982). After a Folin-Wu deproteinization, the liberated glycyl-glycine is derivatized with a borate-buffered (pH 9.3) trinitrobenzenesulfonate solution (60 mmol/L) to form trinitrophenyl-glycylglycine, the absorbance of which is read at 420 nm vs a serum blank. The linear range extends to an activity of more than 900 U/L of serum and the detection limit is less than 4 U/L. The mean activity for serum from 50 blood bank donors and 25 patients with active sarcoidosis was 281 (SD 77) and 693 (SD 81) U/L, respectively. The method demonstrates good precision (CVs less than 2.8%) and correlates well (r = .99) with results from a "high-pressure" liquid chromatographic procedure for determining hippuric acid. In addition, the proposed method is widely applicable, involving only commonly available apparatus.
Language
English
Source (journal)
Clinical chemistry : international journal of laboratory medicine and molecular diagnostics / American Association of Clinical Chemists. - Winston-Salem, N.C., 1955, currens
Publication
Winston-Salem, N.C. : 1983
ISSN
0009-9147 [print]
1530-8561 [online]
Volume/pages
29:7(1983), p. 1399-1403
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
External links
Record
Identification
Creation 29.07.2011
Last edited 02.09.2016