Publication
Title
Screening, identification and quantification of glucosinolates in black radish (**Raphanus sativus** L. niger) based dietary supplements using liquid chromatography coupled with a photodiode array and liquid chromatography - mass spectrometry
Author
Abstract
The glucosinolate profile of black radish (Raphanus sativus L. niger) based dietary supplements has been investigated by HPLC-PDA, LC-ESI-MS/MS and LC-APCI-MS/MS systems. Optimization of the MS/MS parameters and LC conditions was performed using sinigrin reference standard and rapeseed certified reference material (BC190) respectively. An LC-ESI-MS/MS system was used to detect (screen) and identify the naturally occurring intact glucosinolates (GLs). The intact GLs identified were then desulfated and quantified on an HPLC-PDA system as desulfo-glucosinolates (DS-GLs). Prior to quantification, the DS-GLs were identified using an APCI-MS/MS. The HPLC-PDA method performance criteria were evaluated using glucotropaeolin potassium salt. The validated method was applied for the analysis of six dietary supplements. In total, six glucosinolates were identified and quantified in the dietary supplements; glucoraphasatin (0.20.48 mg/g), glucosisaustricin (0.370.91 mg/g), glucoraphenin (0.841.27 mg/g), glucoputrajivin (0.140.28 mg/g), glucosisymbrin (0.700.99 mg/g) and gluconasturtiin (0.060.12 mg/g). Glucoraphenin was the most abundant glucosinolate in all samples.
Language
English
Source (journal)
Journal of chromatography: A. - Amsterdam
Publication
Amsterdam : 2011
ISSN
0021-9673
Volume/pages
1218:28(2011), p. 4395-4405
ISI
000292718800016
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 02.08.2011
Last edited 30.05.2017
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