Title
Screening, identification and quantification of glucosinolates in black radish (**Raphanus sativus** L. niger) based dietary supplements using liquid chromatography coupled with a photodiode array and liquid chromatography - mass spectrometry Screening, identification and quantification of glucosinolates in black radish (**Raphanus sativus** L. niger) based dietary supplements using liquid chromatography coupled with a photodiode array and liquid chromatography - mass spectrometry
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
Amsterdam ,
Subject
Biology
Source (journal)
Journal of chromatography: A. - Amsterdam
Volume/pages
1218(2011) :28 , p. 4395-4405
ISSN
0021-9673
ISI
000292718800016
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
The glucosinolate profile of black radish (Raphanus sativus L. niger) based dietary supplements has been investigated by HPLC-PDA, LC-ESI-MS/MS and LC-APCI-MS/MS systems. Optimization of the MS/MS parameters and LC conditions was performed using sinigrin reference standard and rapeseed certified reference material (BC190) respectively. An LC-ESI-MS/MS system was used to detect (screen) and identify the naturally occurring intact glucosinolates (GLs). The intact GLs identified were then desulfated and quantified on an HPLC-PDA system as desulfo-glucosinolates (DS-GLs). Prior to quantification, the DS-GLs were identified using an APCI-MS/MS. The HPLC-PDA method performance criteria were evaluated using glucotropaeolin potassium salt. The validated method was applied for the analysis of six dietary supplements. In total, six glucosinolates were identified and quantified in the dietary supplements; glucoraphasatin (0.20.48 mg/g), glucosisaustricin (0.370.91 mg/g), glucoraphenin (0.841.27 mg/g), glucoputrajivin (0.140.28 mg/g), glucosisymbrin (0.700.99 mg/g) and gluconasturtiin (0.060.12 mg/g). Glucoraphenin was the most abundant glucosinolate in all samples.
E-info
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000292718800016&DestLinkType=RelatedRecords&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000292718800016&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000292718800016&DestLinkType=CitingArticles&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848
Handle