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Title
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**Plasmodium falciparum** genotyping by microsatellites as a method to distinguish between recrudescent and new infections
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Author
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Abstract
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| In vivo tests for susceptibility to antimalarial drugs require molecular methods to distinguish recrudescence from new infection. The most commonly used DNA markers (merozoite surface proteins [MSPs]) are under immune selective pressure, which might lead to misclassification. We evaluated immunologically neutral microsatellite markers in blood samples collected during a drug efficacy trial in Rwanda. Fifty percent of the infections classified as recrudescent by MSP were classified as new by microsatellite markers. Reciprocally, 23.3% of infections classified as recrudescent by microsatellite markers were identified as new by MSP. In drug efficacy studies, microsatellite markers should complement MSP genotyping to distinguish a recrudescence from a new infection. |
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Language
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English
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Source (journal)
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The American journal of tropical medicine and hygiene. - Baltimore, Md
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Publication
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Baltimore, Md
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2005
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ISSN
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0002-9637
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DOI
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10.4269/AJTMH.2005.73.210
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Volume/pages
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73
:1
(2005)
, p. 210-213
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ISI
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000230451900040
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Full text (Publisher's DOI)
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