Publication
Title
Reverse transcription-polymerase chain reaction construction of plasmid-based, full-length cDNA libraries from **Leishmania infantum** for in vitro expression screening
Author
Abstract
We describe a streamlined reverse transcription-polymerase chain reaction methodology for constructing full-length cDNA libraries of trypanosomatids on the basis of conserved sequences located at the 5' and 3'ends of trans-spliced mRNAs. The amplified cDNA corresponded to full-length messengers and was amenable to in vitro expression. Fractionated libraries could be rapidly constructed in a plasmid vector by the TA cloning method (Invitrogen). We believe this is useful when there are concerns over the use of restriction enzymes and phage technology as well as in cases where expression of proteins in their native conformation is desired.
Language
English
Source (journal)
Memorias do Instituto Oswaldo Cruz. - Rio de Janeiro
Publication
Rio de Janeiro : 2003
ISSN
0074-0276
Volume/pages
98:4(2003), p. 477-480
ISI
000183993100008
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 04.08.2011
Last edited 14.10.2017