Title
Reverse transcription-polymerase chain reaction construction of plasmid-based, full-length cDNA libraries from **Leishmania infantum** for in vitro expression screening Reverse transcription-polymerase chain reaction construction of plasmid-based, full-length cDNA libraries from **Leishmania infantum** for in vitro expression screening
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
Rio de Janeiro ,
Subject
Human medicine
Source (journal)
Memorias do Instituto Oswaldo Cruz. - Rio de Janeiro
Volume/pages
98(2003) :4 , p. 477-480
ISSN
0074-0276
ISI
000183993100008
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
We describe a streamlined reverse transcription-polymerase chain reaction methodology for constructing full-length cDNA libraries of trypanosomatids on the basis of conserved sequences located at the 5' and 3'ends of trans-spliced mRNAs. The amplified cDNA corresponded to full-length messengers and was amenable to in vitro expression. Fractionated libraries could be rapidly constructed in a plasmid vector by the TA cloning method (Invitrogen). We believe this is useful when there are concerns over the use of restriction enzymes and phage technology as well as in cases where expression of proteins in their native conformation is desired.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/e4d36c/fb34b886.pdf
E-info
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000183993100008&DestLinkType=RelatedRecords&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000183993100008&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848