|
Title
|
|
|
|
American tegumentary leishmaniasis : direct species identification of **Leishmania** in non-invasive clinical samples
|
|
|
Author
|
|
|
|
|
|
|
Abstract
|
|
|
|
| Species identification is highly relevant for improved prognosis and adequate treatment of American tegumentary leishmaniasis (ATL). PCR-based methods are available for this purpose but should be simplified to improve accessibility. As a first step in this process, this paper describes a simplified protocol for collection of clinical samples. Using samples from 44 Bolivian patients with confirmed ATL, we demonstrated that hsp70 PCRRFLP on skin scrapings collected with a tooth pick allowed identification of the parasite species with a sensitivity of 95% and specificity of 100%. Our method should greatly facilitate individual patient management and epidemiological surveillance of ATL. |
|
|
|
Language
|
|
|
|
English
|
|
|
Source (journal)
|
|
|
|
Transactions of the Royal Society of Tropical Medicine and Hygiene. - London
|
|
|
Publication
|
|
|
|
London
:
2007
|
|
|
ISSN
|
|
|
|
0035-9203
|
|
|
Volume/pages
|
|
|
|
101
:4
(2007)
, p. 368-371
|
|
|
ISI
|
|
|
|
000245059100008
|
|
|
Full text (Publisher's DOI)
|
|
|
|
|
|