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Title
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American tegumentary leishmaniasis : direct species identification of **Leishmania** in non-invasive clinical samples
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Author
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Abstract
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| Species identification is highly relevant for improved prognosis and adequate treatment of American tegumentary leishmaniasis (ATL). PCR-based methods are available for this purpose but should be simplified to improve accessibility. As a first step in this process, this paper describes a simplified protocol for collection of clinical samples. Using samples from 44 Bolivian patients with confirmed ATL, we demonstrated that hsp70 PCRRFLP on skin scrapings collected with a tooth pick allowed identification of the parasite species with a sensitivity of 95% and specificity of 100%. Our method should greatly facilitate individual patient management and epidemiological surveillance of ATL. |
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Language
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English
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Source (journal)
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Transactions of the Royal Society of Tropical Medicine and Hygiene. - London
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Publication
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London
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2007
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ISSN
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0035-9203
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DOI
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10.1016/J.TRSTMH.2006.06.009
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Volume/pages
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101
:4
(2007)
, p. 368-371
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ISI
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000245059100008
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Full text (Publisher's DOI)
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