Title
American tegumentary leishmaniasis : direct species identification of **Leishmania** in non-invasive clinical samples American tegumentary leishmaniasis : direct species identification of **Leishmania** in non-invasive clinical samples
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
Subject
Human medicine
Source (journal)
Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume/pages
101(2007) :4 , p. 368-371
ISSN
0035-9203
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Species identification is highly relevant for improved prognosis and adequate treatment of American tegumentary leishmaniasis (ATL). PCR-based methods are available for this purpose but should be simplified to improve accessibility. As a first step in this process, this paper describes a simplified protocol for collection of clinical samples. Using samples from 44 Bolivian patients with confirmed ATL, we demonstrated that hsp70 PCRRFLP on skin scrapings collected with a tooth pick allowed identification of the parasite species with a sensitivity of 95% and specificity of 100%. Our method should greatly facilitate individual patient management and epidemiological surveillance of ATL.