Title
Pathogen genotyping in polyclonal infections : application of a fluorogenic polymerase-chain-reaction assay in malaria
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
Chicago, Ill. ,
Subject
Human medicine
Source (journal)
The journal of infectious diseases. - Chicago, Ill.
Volume/pages
188(2003) :8 , p. 1245-1249
ISSN
0022-1899
ISI
000185850100021
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Pathogen genotyping of polyclonal infections is limited by 2 major drawbacks: (1) how to establish whether multiple mutations detected in 1 gene belong to the same clone and (2) how to evaluate the proportion of different genotypes in a given sample. For drug-resistance genotyping in Plasmodium falciparum malaria, we address these problems by using a fluorogenic assay that combines fluorescence-resonance energy transfer, between fluorophores present on a probe and a polymerase-chain-reaction primer, and a melt-curve analysis. We demonstrate that this tool allows a more accurate insight into the P. falciparum populations present in complex biological samples.
E-info
https://repository.uantwerpen.be/docman/iruaauth/f07e4c/95fb5776e7d.pdf
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