Publication
Title
Pathogen genotyping in polyclonal infections : application of a fluorogenic polymerase-chain-reaction assay in malaria
Author
Abstract
Pathogen genotyping of polyclonal infections is limited by 2 major drawbacks: (1) how to establish whether multiple mutations detected in 1 gene belong to the same clone and (2) how to evaluate the proportion of different genotypes in a given sample. For drug-resistance genotyping in Plasmodium falciparum malaria, we address these problems by using a fluorogenic assay that combines fluorescence-resonance energy transfer, between fluorophores present on a probe and a polymerase-chain-reaction primer, and a melt-curve analysis. We demonstrate that this tool allows a more accurate insight into the P. falciparum populations present in complex biological samples.
Language
English
Source (journal)
The journal of infectious diseases. - Chicago, Ill.
Publication
Chicago, Ill. : 2003
ISSN
0022-1899
Volume/pages
188:8(2003), p. 1245-1249
ISI
000185850100021
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 04.08.2011
Last edited 28.06.2017