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Title
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Pathogen genotyping in polyclonal infections : application of a fluorogenic polymerase-chain-reaction assay in malaria
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Author
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Abstract
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| Pathogen genotyping of polyclonal infections is limited by 2 major drawbacks: (1) how to establish whether multiple mutations detected in 1 gene belong to the same clone and (2) how to evaluate the proportion of different genotypes in a given sample. For drug-resistance genotyping in Plasmodium falciparum malaria, we address these problems by using a fluorogenic assay that combines fluorescence-resonance energy transfer, between fluorophores present on a probe and a polymerase-chain-reaction primer, and a melt-curve analysis. We demonstrate that this tool allows a more accurate insight into the P. falciparum populations present in complex biological samples. |
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Language
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English
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Source (journal)
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The journal of infectious diseases. - Chicago, Ill.
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Publication
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Chicago, Ill.
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2003
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ISSN
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0022-1899
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DOI
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10.1086/378521
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Volume/pages
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188
:8
(2003)
, p. 1245-1249
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ISI
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000185850100021
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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