Title
Fluorogenic assay for molecular typing of the **Leishmania donovani** complex : taxonomic and clinical applicationsFluorogenic assay for molecular typing of the **Leishmania donovani** complex : taxonomic and clinical applications
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Research group
Department of Biomedical Sciences
Publication type
article
Publication
Chicago, Ill.,
Subject
Human medicine
Source (journal)
The journal of infectious diseases. - Chicago, Ill.
Volume/pages
192(2005):4, p. 685-692
ISSN
0022-1899
ISI
000231019500018
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
We describe a new fluorogenic assay for the identification of species and intraspecies groups within the Leishmania donovani complex. The assay combined (1) 2 polymerase chain reactions targeting the 2 cysteine proteinase b isogenes and (2) a fluorescence-resonance energy transfer/melting curve analysis of the polymorphisms within a 31-nt region. All strains within the L. donovani complex were distinguished from L. tropica, L. major, and L. aethiopica, and 5 distinct groups were identified within the L. donovani complex. Discrepancies were observed with the present taxonomy on the basis of isoenzyme analysis and concerned East African strains, which suggests the need for a systematic reevaluation of the taxonomy. The capacity to type parasites directly from clinical samples was demonstrated with blood and bone marrow samples. This rapid and high-throughput alternative for molecular diagnosis and epidemiological studies of visceral leishmaniasis could be adapted for use with other Leishmania species.
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