Title
Culture-independent species typing of neotropical **Leishmania** for clinical validation of a PCR-based assay targeting heat shock protein 70 genes Culture-independent species typing of neotropical **Leishmania** for clinical validation of a PCR-based assay targeting heat shock protein 70 genes
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
Washington, D.C. ,
Subject
Human medicine
Source (journal)
Journal of clinical microbiology. - Washington, D.C.
Volume/pages
42(2004) :5 , p. 2294-2297
ISSN
0095-1137
ISI
000221424100077
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
PCR-restriction fragment length polymorphism analysis of heat shock protein 70 genes discriminates most neotropical Leishmania species, as well as Trypanosoma cruzi. The assay, combined with capillary electrophoresis in a microchip device, may be applied directly on clinical samples with a high sensitivity, hence supporting clinical and epidemiological monitoring of leishmaniasis.
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