Title
The sensitivity of clinical isolates of **Leishmania** from Peru and Nepal to miltefosine
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
Baltimore, Md ,
Subject
Human medicine
Source (journal)
The American journal of tropical medicine and hygiene. - Baltimore, Md
Volume/pages
73(2005) :2 , p. 272-275
ISSN
0002-9637
ISI
000231272700010
Carrier
E
Target language
English (eng)
Abstract
Clinical isolates of Leishmania, from visceral leishmaniasis (VL) cases in Nepal and from cutaneous leishmaniasis (CL) cases in Peru, were cultured using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to type species and strain. Promastigotes from 38 isolates, within eight passages from isolation, were used to infect mouse peritoneal macrophage cultures in vitro, and the amastigote sensitivity to miltefosine was determined. The concentration required to kill 50% of intracellular amastigotes from Nepalese VL isolates, all typed as Leishmania (L.) donovani (N = 24) from both Sbv responders and nonresponders, ranged from 8.7 to 0.04 μg/mL. In contrast, the concentration required to kill 50% intracellular amastigotes from isolates from Peru, typed as L.(V.) braziliensis (N = 8), was > 30 to 8.4 μg/mL, L.(V.) guyanensis (N = 2) > 30 to 1.9 μg/mL, L.(L.) mexicana (N = 1) > 30 μg/mL, and L. (V.) lainsoni (N = 4) was 3.4 to 1.9 μg/mL. This demonstrates a notable difference in the intrinsic sensitivity of Leishmania species to miltefosine in vitro. If this model can be correlated to therapeutic outcome, it may have implications for the interpretation of clinical trials.
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