Publication
Title
The sensitivity of clinical isolates of **Leishmania** from Peru and Nepal to miltefosine
Author
Abstract
Clinical isolates of Leishmania, from visceral leishmaniasis (VL) cases in Nepal and from cutaneous leishmaniasis (CL) cases in Peru, were cultured using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to type species and strain. Promastigotes from 38 isolates, within eight passages from isolation, were used to infect mouse peritoneal macrophage cultures in vitro, and the amastigote sensitivity to miltefosine was determined. The concentration required to kill 50% of intracellular amastigotes from Nepalese VL isolates, all typed as Leishmania (L.) donovani (N = 24) from both Sbv responders and nonresponders, ranged from 8.7 to 0.04 μg/mL. In contrast, the concentration required to kill 50% intracellular amastigotes from isolates from Peru, typed as L.(V.) braziliensis (N = 8), was > 30 to 8.4 μg/mL, L.(V.) guyanensis (N = 2) > 30 to 1.9 μg/mL, L.(L.) mexicana (N = 1) > 30 μg/mL, and L. (V.) lainsoni (N = 4) was 3.4 to 1.9 μg/mL. This demonstrates a notable difference in the intrinsic sensitivity of Leishmania species to miltefosine in vitro. If this model can be correlated to therapeutic outcome, it may have implications for the interpretation of clinical trials.
Language
English
Source (journal)
The American journal of tropical medicine and hygiene. - Baltimore, Md
Publication
Baltimore, Md : 2005
ISSN
0002-9637
Volume/pages
73:2(2005), p. 272-275
ISI
000231272700010
UAntwerpen
Faculty/Department
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 04.08.2011
Last edited 25.06.2017