Title
**Trypanosoma cruzi** : variability of stocks from Colombia determined by molecular karyotype and minicircle Southern blot analysis **Trypanosoma cruzi** : variability of stocks from Colombia determined by molecular karyotype and minicircle Southern blot analysis
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
New York ,
Subject
Human medicine
Source (journal)
Experimental parasitology. - New York
Volume/pages
113(2006) :1 , p. 62-66
ISSN
0014-4894
ISI
000237440600010
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Nineteen Trypanosoma cruzi stocks, most of them of wild origin, and four Trypanosoma rangeli stocks from Colombia were analysed by molecular karyotype analysis with cloned DNA cruzipain as the probe. Another 27 cloned stocks of T. cruzi from different geographic areas of South America were used as reference for T. cruzi lineages. Phenetic analysis of chromosome size polymorphism demonstrated a great variability of Colombian T. cruzi stocks, suggesting that most belong to lineage I, although two of them belong to lineage II. The 2 lineage II T. cruzi, 17 T. cruzi lineage I, and 3 T. rangeli stocks from Colombia were studied further by Southern blot analysis with a panel of kinetoplast DNA minicircle probes. Hybridisation results indicate that the two T. cruzi II stocks are genetically distant from each other and from T. cruzi lineages IIb, IId, and IIe from Chile. Finally, T. cruzi minicircle probes do not cross-hybridise in any stringency condition tested with T. rangeli minicircles, a clear indication that these parasites can be easily distinguished by this method.
E-info
https://repository.uantwerpen.be/docman/iruaauth/781c56/f5fa32678e7.pdf
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