Publication
Title
Rapid, broadband two-photon-excited fluorescence spectroscopy and its application to red-emitting secondary reference compounds
Author
Abstract
We present a methodology for rapidly acquiring broadband two-photon absorption (2PA) spectra by means of a compact two-channel femtosecond fluorescence excitation apparatus. This technique is insensitive to differences in excitation and collection geometries as well as detection efficiencies between the sample and reference, in addition to variations in average power, pulse duration and spatial beam profile, as it utilizes sequential measurement of the sample and reference in each of the two cell positions. Our approach eliminates the need to determine the fluorescence quantum yields of the sample and reference, as it allows measurement of emission from samples at a common specified wavelength. These attributes allow for acquisition of 2PA spectra with an estimated accuracy of ± 15% (limited almost exclusively by the uncertainty in the 2PA cross section for the reference standards) over an excitation range of 550-1600 nanometers with a typical time per spectrum of ~30-60 minutes. We have applied this technique to determine the 2PA spectra of six commercially available organic dyes over a wide range of excitation wavelengths (670-1600 nm), which can be used as secondary reference standards emitting in the red and near-infrared spectral region (600-1000 nm). We have also characterized some of these compounds using the femtosecond-pulsed Z-scan method and found very good agreement with the fluorescence-based measurements.
Language
English
Source (journal)
Optical materials express
Publication
2011
ISSN
2159-3930
Volume/pages
1:4(2011), p. 551-563
ISI
000299048100004
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 17.10.2011
Last edited 07.08.2017
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