Title
The OPFOS microscopy family : high-resolution optical sectioning of biomedical specimens The OPFOS microscopy family : high-resolution optical sectioning of biomedical specimens
Author
Faculty/Department
Faculty of Sciences. Physics
Publication type
article
Publication
Subject
Physics
Biology
Human medicine
Source (journal)
Anatomy Research International
Volume/pages
(2012) , p. 1-9
ISSN
2090-2743
Article Reference
206238
Carrier
E-only publicatie
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
We report on the recently emerging (laser) light-sheet-based fluorescence microscopy field (LSFM). The techniques used in this field allow to study and visualize biomedical objects nondestructively in high resolution through virtual optical sectioning with sheets of laser light. Fluorescence originating in the cross-section of the sheet and sample is recorded orthogonally with a camera. In this paper, the first implementation of LSFM to image biomedical tissue in three dimensionsorthogonal-plane fluorescence optical sectioning microscopy (OPFOS)is discussed. Since then many similar and derived methods have surfaced, (SPIM, ultramicroscopy, HR-OPFOS, mSPIM, DSLM, TSLIM, etc.) which we all briefly discuss. All these optical sectioning methods create images showing histological detail. We illustrate the applicability of LSFM on several specimen types with application in biomedical and life sciences.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/d6d489/60aafa76.pdf
Handle