Publication
Title
The OPFOS microscopy family : high-resolution optical sectioning of biomedical specimens
Author
Abstract
We report on the recently emerging (laser) light-sheet-based fluorescence microscopy field (LSFM). The techniques used in this field allow to study and visualize biomedical objects nondestructively in high resolution through virtual optical sectioning with sheets of laser light. Fluorescence originating in the cross-section of the sheet and sample is recorded orthogonally with a camera. In this paper, the first implementation of LSFM to image biomedical tissue in three dimensionsorthogonal-plane fluorescence optical sectioning microscopy (OPFOS)is discussed. Since then many similar and derived methods have surfaced, (SPIM, ultramicroscopy, HR-OPFOS, mSPIM, DSLM, TSLIM, etc.) which we all briefly discuss. All these optical sectioning methods create images showing histological detail. We illustrate the applicability of LSFM on several specimen types with application in biomedical and life sciences.
Language
English
Source (journal)
Anatomy Research International
Publication
2012
ISSN
2090-2743
Volume/pages
(2012), p. 1-9
Article Reference
206238
Medium
E-only publicatie
Full text (Publishers DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Record
Identification
Creation 08.11.2011
Last edited 12.09.2015
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