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Title
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Electrochemical determination of hydrogen peroxide with cytochrome c peroxidase and horse heart cytochrome c entrapped in a gelatin hydrogel
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Author
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Abstract
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| A novel and versatile method, based on a membrane-free enzyme electrode in which both the enzyme and a mediator protein are entrapped in a gelatine hydrogel was developed for the fabrication of biosensors. As a proof of principle, we prepared a hydrogen peroxide biosensor by successfully entrapping both horse heart cytochrome c (HHC) and Saccharomyces cerevisae cytochrome c peroxidase (CCP) in a gelatin matrix which is immobilized on a gold electrode. This electrode was first pretreated with 6-mercaptohexanol. The biosensor displayed a rapid response and an expanded linear response range from 0 to 0.3 mM (R = 0.987) with a detection limit of 1 × 10− 5 M in a HEPES buffer solution (pH 7.0). This method of encapsulation is now further investigated for industrial biosensor applications. |
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Language
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English
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Source (journal)
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Bioelectrochemistry: an international journal devoted to electrochemical aspects of biology and biological aspects of electrochemistry. - Oxford
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Publication
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Oxford
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Elsevier
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2012
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ISSN
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1567-5394
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DOI
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10.1016/J.BIOELECHEM.2011.07.001
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Volume/pages
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83
(2012)
, p. 15-18
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ISI
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000297962500003
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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