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Title
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Serial day rapid kindling is an effective tool in screening the anti-epileptic properties of topiramate
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Author
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Abstract
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| Introduction In this study, a serial day rapid kindling protocol was used to fully kindle rats in a matter of days. Subsequently, the anticonvulsant profile of a relatively new anti-epileptic drug, topiramate, was evaluated in a cross-over design to further validate this rapid kindling model. Methods Rats were kindled during three consecutive days, according to the serial day rapid kindling protocol. Topiramate was tested at a dose of 100 mg/kg, i.p., over the next 2 days using a cross-over design. The stability of the kindled state was evaluated in all rats during two retest paradigms. During the drug-testing procedure, rats received a single i.p. injection of either topiramate or verhicle. Starting 1 h later the rats received additional kindling stimulations during which their response was measured. Results Serial day rapid kindling induced a long lasting and stable fully kindled state that allowed for the anti-epileptic drug screening procedure. Topiramate reduced both the afterdischarge duration and ameliorated seizure semiology in the kindled rats. Discussion Serial day rapid kindling provided a tool to rapidly kindle rats in 3 days. Using a cross-over design, clear indications on anti-epileptic activity of a given drug can be determined using few laboratory animals. |
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Language
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English
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Source (journal)
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Seizure: European journal of epilepsy. - London
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Publication
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London
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2007
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ISSN
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1059-1311
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DOI
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10.1016/J.SEIZURE.2007.04.013
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Volume/pages
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16
:7
(2007)
, p. 620-626
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ISI
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000249651800008
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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