Title
Basophil activation reveals divergent patient-specific responses to thermally processed peanuts Basophil activation reveals divergent patient-specific responses to thermally processed peanuts
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Barcelona ,
Subject
Human medicine
Source (journal)
Journal of investigational allergology & clinical immunology : official organ of the International Association of Asthmology (INTERASMA) and Sociedad Latinoamericana de Alergia e Inmunologia / International Association of Asthmology. - Barcelona
Volume/pages
21(2011) :7 , p. 527-531
ISSN
1018-9068
ISI
000298935200005
Carrier
E
Target language
English (eng)
Affiliation
University of Antwerp
Abstract
Introduction: The impact of processing on the allergenicity of peanut (Arachis hypogaea) proteins has traditionally been studied using immunoglobulin (Ig) E binding assay. However, as this technique does not assess the potential of an allergen to trigger basophils and mast cells, studies based on it can hardly be considered complete. We evaluated the effect of processing on peanut allergenicity using fl ow-cytometric quantifi cation of in vitro basophil activation (basophil activation test [BAT]). Patients and Methods: Basophils from 10 patients with severe peanut allergy and 3 peanut-tolerant individuals were stimulated with extracts from 5 raw and thermally processed peanut varieties. Data were compared using protein staining (sodium dodecyl sulfate-polyacrylamide gel electrophoresis [SDS-PAGE]) and IgE immunoblotting. Results: Stimulation with different extracts resulted in patient-dependent and variety-dependent effects on basophil activation. SDS-PAGE revealed a considerable loss of identifi able bands, especially for the South Africa Common Natal, Argentina Runner, and US Virginia varieties. The results of IgE immunoblotting in patients were similar, irrespective of the responses observed in the BAT. Conclusions: The impact of thermal processing on the capacity of peanuts to trigger basophils seems highly divergent between patients and cannot be predicted using SDS-PAGE or IgE binding. BAT can be considered a complementary tool for the evaluation of food allergenicity.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/97f85e/2d73b2a5.pdf
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