Title
EPR investigation of the role of B10 phenylalanine in neuroglobin : evidence that B10Phe mediates structural changes in the heme region upon disulfide-bridge formation EPR investigation of the role of B10 phenylalanine in neuroglobin : evidence that B10Phe mediates structural changes in the heme region upon disulfide-bridge formation
Author
Faculty/Department
Faculty of Sciences. Physics
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
New York, N.Y. ,
Subject
Chemistry
Biology
Pharmacology. Therapy
Source (journal)
Journal of inorganic biochemistry. - New York, N.Y.
Volume/pages
105(2011) :9 , p. 1131-1137
ISSN
0162-0134
ISI
000294085500003
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
The function of neuroglobin, a member of the vertebrate globin family, is still unknown. In human neuroglobin (NGB), the formation of a disulfide bridge between the CysCD7 and CysD5 is known to affect the heme environment and its ligand-binding kinetics. Here, we show by means of EPR that the PheB10 residue plays a key role in transmitting the structural information from the disulfide bridge to the heme-pocket region. While formation of a disulfide bridge in ferric wild-type NGB leads to a considerable change of its EPR parameters, only minor changes are observed in the case of ferric PheB10Leu NGB. Furthermore, wild-type NGB is found to be much more stable in the presence of H2O2 than its PheB10Leu or its HisE7Leu mutants. While tyrosyl radicals are induced in HisE7Leu NGB by the addition of H2O2, this is not the case for wild-type and PheB10Leu NGB. The results will be discussed in terms of the protein's putative functions.
E-info
https://repository.uantwerpen.be/docman/iruaauth/bbd5f3/72cf93783f9.pdf
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