Publication
Title
Detection, localization, and conformational analysis of single polysaccharide molecules on live bacteria
Author
Abstract
The nanoscale exploration of microbes using atomic force microscopy (AFM) is an exciting, rapidly evolving research field. Here, we show that single-molecule force spectroscopy is a valuable tool for the localization and conformational analysis of individual polysaccharides on live bacteria. We focus on the clinically important probiotic bacterium Lactobacillus rhamnosus GG, demonstrating the power of AFM to reveal the coexistence of polysaccharide chains of different nature on the cell surface. Applicable to a wide variety of cells, this single molecule method offers exciting prospects for analyzing the heterogeneity and diversity of macromolecules constituting cell membranes and cell walls.
Language
English
Source (journal)
ACS nano
Publication
2008
ISSN
1936-0851
Volume/pages
2:9(2008), p. 1921-1929
ISI
000259450300026
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 02.01.2012
Last edited 15.07.2017