Title
Detection, localization, and conformational analysis of single polysaccharide molecules on live bacteriaDetection, localization, and conformational analysis of single polysaccharide molecules on live bacteria
Author
Faculty/Department
Faculty of Sciences. Bioscience Engineering
Publication type
article
Publication
Subject
Biology
Human medicine
Engineering sciences. Technology
Source (journal)
ACS nano
Volume/pages
2(2008):9, p. 1921-1929
ISSN
1936-0851
ISI
000259450300026
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
The nanoscale exploration of microbes using atomic force microscopy (AFM) is an exciting, rapidly evolving research field. Here, we show that single-molecule force spectroscopy is a valuable tool for the localization and conformational analysis of individual polysaccharides on live bacteria. We focus on the clinically important probiotic bacterium Lactobacillus rhamnosus GG, demonstrating the power of AFM to reveal the coexistence of polysaccharide chains of different nature on the cell surface. Applicable to a wide variety of cells, this single molecule method offers exciting prospects for analyzing the heterogeneity and diversity of macromolecules constituting cell membranes and cell walls.
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