Title
Characterization of MabA, a modulator of **Lactobacillus rhamnosus** GG adhesion and biofilm formation Characterization of MabA, a modulator of **Lactobacillus rhamnosus** GG adhesion and biofilm formation
Author
Faculty/Department
Faculty of Sciences. Bioscience Engineering
Publication type
article
Publication
Amsterdam ,
Subject
Biology
Human medicine
Engineering sciences. Technology
Source (journal)
FEMS immunology and medical microbiology / Federation of European Microbiological Societies. - Amsterdam
Volume/pages
59(2010) :3 , p. 386-398
ISSN
0928-8244
ISI
000279900400016
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
The probiotic Lactobacillus rhamnosus GG, first isolated from healthy human gut microbiota, has been reported to adhere very well to components of the intestinal mucosa, thereby enabling transient colonization of the gastrointestinal tract (GIT). In a search for the genes responsible for the good adherence capacity of this strain, a genomic region encoding a protein with homology to putative adhesion proteins (LGG_01865) and its putative regulator (LGG_01866) was identified. The sequence of the L. rhamnosus GG LGG_01865 encodes a polypeptide of 2419 amino acid residues containing 26 repetitive DUF1542 domains and a C-terminal LPxTG cell wall-anchoring motif. Phenotypic analyses of a dedicated LGG_01865 knockout mutant revealed a reduced biofilm formation capacity on abiotic surfaces and decreased adhesion to intestinal epithelial cells and tissues of the murine GIT. This suggests a modulating role for LGG_01865 in L. rhamnosus GGhost interactions. Therefore, we propose a new name for LGG_01865, i.e. MabA, modulator of adhesion and biofilm. Expression analysis indicated that LGG_01866 plays a conditional role in the regulation of LGG_01865 expression, i.e. when cells are grown under conditions of sugar starvation.
E-info
https://repository.uantwerpen.be/docman/iruaauth/0b1c7e/25232d33e5f.pdf
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